Correlation of glioma proliferation assessed by flow cytometry with 99mTc-Tetrofosmin SPECT uptake

Alexiou, George A.; Tsiouris, Spyridon; Vartholomatos, George; Fotakopoulos, George; Papadopoulos, Athanasios; Kyritsis, Athanasios P.; Voulgaris, Spyridon; Fotopoulos, Andreas D.

doi:10.1016/j.clineuro.2009.08.009

« Previous Next »Clinical Neurology and Neurosurgery
Volume 111, Issue 10 , Pages 808-811, December 2009

Correlation of glioma proliferation assessed by flow cytometry with ^99mTc-Tetrofosmin SPECT uptake☆

George A. Alexiou
- Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece
- Corresponding author at: P.O. Box 103, Neohoropoulo, Ioannina, 455 00, Greece. Tel.: +30 26510 48795; fax: +30 6948 525134.
Spyridon Tsiouris
- Nuclear Medicine Department, University Hospital of Ioannina, Ioannina, Greece
George Vartholomatos
- Haematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, Ioannina, Greece
George Fotakopoulos
- Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece
Athanasios Papadopoulos
- Nuclear Medicine Department, University Hospital of Ioannina, Ioannina, Greece
Athanasios P. Kyritsis
- Department of Neurology, Neurosurgical Institute, University Hospital of Ioannina, Ioannina, Greece
Spyridon Voulgaris
- Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece
Andreas D. Fotopoulos
- Nuclear Medicine Department, University Hospital of Ioannina, Ioannina, Greece

Received 19 February 2009; received in revised form 13 June 2009; accepted 9 August 2009.

Abstract

Objectives

Brain single-photon emission computed tomography (SPECT) has been proposed as a potentially useful modality for the metabolic assessment of various brain tumors.

Material and methods

In a 10-patient prospective pilot study we evaluated whether ^99mTc-Tetrofosmin (^99mTc-TF) uptake correlates with glioma proliferative activity assessed by flow cytometric analysis. ^99mTc-TF brain SPECT was performed shortly before surgical tumor excision.

Results

Eight patients were diagnosed with glioblastoma multiform and 2 with anaplastic astrocytoma. All tumors were aneuploid. We found a significant positive linear correlation between ^99mTc-TF uptake and percentage of tumor cells on the S-phase of the cell cycle (r=0.92, P=0.001).